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KMID : 0379119890170020057
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Korean Journal of Mycology
1989 Volume.17 No. 2 p.57 ~ p.65
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Synergistic Effect of Substrates on the Biosynthesis of Cellulase and Xylanase Complexes from Aspergillus nidulans
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Abstract
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The effect of various cellulosic and hemicellulosic substrates on the induction of cellulase and xylanase complexes in Aapergillus nidulans was investigated. The most efficient substrates for the induction of cellulase and xylanase complexes were carboxymethylcellulose for endoglucanase, cellobiose for β-glucosidase, and xylan for endoxylanase and β-xylosidase, respectively. However, the mixtures of these substrates, especially CMC-xylan and CMC-xylan-laminarin mixture, were much more effective not only for the enhancement of the biosynthesis of all the cellulase and xylanase complexes but also for the balanced production of these enzyme components than individual substrate. The polyacrylamide gel electrophoresis followed by activity staining showed the variation in the patterns and relative intensity of β-glucoeidase, endoglucanase and endoxylanase components in individual enzyme preparations from A. nidulans cultures grown on different substrates. These results suggest that the biosynthesis is of cellulase and xylanase systems in A. nidulans is regulated in coordination at the level of induction.
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